We have also developed effective methods for detecting some enzymes in live cells. Our RediPlate product line includes enzyme substrates predispensed in 96-well plates for high-throughput applications, along with the appropriate reference standards and other reaction components. We prepare substrates for detecting very low levels of enzymatic activity in fixed cells, tissues, cell extracts and purified preparations, as well as substrates for enzyme-linked immunosorbent assays (ELISAs). We offer a large assortment of fluorogenic and chromogenic enzyme substrates, which are described in the following sections. Chapter 23-Antifades and Other Tools for Fluorescence Applications.Chapter 22-Probes for Membrane Potential.Chapter 21-Indicators for Na+, K+, Cl– and Miscellaneous Ions.Chapter 19-Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions.Chapter 18-Probes for Reactive Oxygen Species, Including Nitric Oxide.Chapter 17-Probes for Signal Transduction.Chapter 16-Probes for Endocytosis, Receptors and Ion Channels.Chapter 15-Assays for Cell Viability, Proliferation and Function.Chapter 14-Fluorescent Tracers of Cell Morphology and Fluid Flow.Chapter 13-Probes for Lipids and Membranes.Chapter 11-Probes for Cytoskeletal Proteins. Chapter 10-Enzyme Substrates and Assays.Chapter 9-Protein Detection and Analysis.Chapter 8-Nucleic Acid Detection and Analysis.
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